Selection and analysis of candidate genes putatively involved in malonyl-CoA supply for flavonoid biosynthesis. A, KAT5 coexpression network. The top 50 genes coexpressing with KAT5 were selected based on the highest Spearman rank correlation coefficients obtained from the web platform ATTED-II (Obayashi et al., 2018). A pairwise correlation threshold of 0.55 was applied as a cutoff to establish the edges between pairs of nodes (genes). Genes that were not connected to any neighbor within the network were excluded from further analysis. Green circles represent the characterized flavonoid-related genes, purple circles the lipid-related genes, and red circles the candidate genes putatively involved in fatty acid degradation and production of malonyl-CoA selected for functional characterization. B, Promoter analysis of candidate genes. A 2.0-kb region upstream to the transcription start site (TSS) of each candidate gene was analyzed using the web resource PlantPAN 3.0 (Chow et al., 2019). Gray diamonds indicate predicted flavonoid-related MYB111 (PFG3) binding motifs.