Overexpression of OsPID stimulates the formation of stigmas and represses the formation of stamens. A, Florets of ospid-1 plants complemented with an OsPID genomic fragment. A floret with palea and lemma closed (left), a floret with two stigmas (middle), and a floret with three stigmas (right) are shown. B, OsPID overexpression plants developed more than three stigmas and fewer than six stamens. C, Quantitative analysis of stigma numbers in wild-type (wt) and OsPID-overexpressing (OsPID-OE) flowers. Data shown are from three independent OsPID overexpression lines. The inset graphic markers 2, 3, and 4 refer to stigma numbers. D, Overexpression of OsPID leads to fewer stamens. The inset graphic markers 4, 5, and 6 refer to stamen numbers. Total numbers of the flowers analyzed in C and D are 47, 73, 52, and 25 for the wild type, OsPID-OE1, OsPID-OE2, and OsPID-OE3, respectively. Bars = 1 mm in A and B.