Figure 8.
Characterization of pmt1 pmt2 pmt3 triple mutants. A, Levels of radiolabeled PCho and PC in the 14-d-old seedlings of the wild type and pmt1-1 pmt2-1 pmt3-2 following [14C]Etn labeling. Radioactive intensity (arbitrary unit) was normalized to the fresh weight of seedlings. Data are mean ±sd from three biological replicates. N.D., not detectable. B, Phenotype of the 14-d-old seedlings of the wild type, pmt1-1 pmt2-1 pmt3-2 and pmt1-2 pmt2-2 pmt3-1 with or without removing the seed coat before germination. A representative image was shown for each condition (n > 30). Bars = 2mm.