GH1-HMGA1 is involved in DNA damage repair. A, DNA damage sensitivity assay with seedlings treated with 100 Gy irradiation (IR). The DNA damage-sensitive mutant ku80 xrcc1 xpf was used as a control. Error bars indicate the se of three biological replicates with 50 seedlings each. B, DNA damage sensitivity assay with seedlings treated with 40 µm MMC. The RAD51-GFP line was used as a control (Da Ines et al., 2013). Error bars indicate the se of three biological replicates with 50 seedlings each. C, Representative plates showing control plants and plants grown 14 d in the presence of 40 µm MMC. D, DNA damage sensitivity assay of seedlings treated with 0.5 mm hydroxyurea (HU). Quantification is shown for the root length of plants grown on control medium or on medium supplemented with 0.5 mm hydroxyurea (n = 30). Black circles represent raw data. Error bars indicate the se. The atr mutant line was used as a positive control for hydroxyurea sensitivity. WT, Wild type.
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