Phenotypes of the mutants bta1l, cct1, and pect1 grown at low temperature. A, Number of cells for EV and the indicated mutants after 3 d of culture. B to E, Comparison of relative polar lipid composition (B) and fatty acid profile of PC (C), PE (D), and DGTS (E) in EV, bta1l, cct1, and pect1. F and G, Molar ratio of 20:5 in extraplastidic (F) and plastidic (G) membrane lipids. Data represent the mean ± sd of three biologically independent experiments. Statistical significance was determined with Dunnett’s test (compared with EV): *, P < 0.05; **, P < 0.01; ***, P < 0.001. SQDG, Sulfoquinovosyldiacylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol.