Membrane lipid analysis of the BTA1L overexpression line. A, Comparison of membrane lipid composition between EV and OEBTA1L. B to D, Fatty acid profiles for DGTS (B), MGDG (C), and DGDG (D). Data represent the mean ± sd of three biologically independent experiments. Statistical significance was determined with Tukey’s test and is indicated by letters at the top. E, Distribution of 20:5 within the eight classes of membrane lipid after 2 d of culture. Data represent the mean ± sd of three biologically independent experiments. Statistical significance was determined with the two-tailed Student’s t test: *, P < 0.05; **, P < 0.01. SQDG, Sulfoquinovosyldiacylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol.