Knockout of WDL5 partially suppresses ethylene-promoted microtubule reassembly in response to salt stress. A, Cortical microtubules in cotyledon pavement cells from wild-type (YFP-tubulin) and mutant wdl5-1 (YFP-tubulin) seedlings treated with 125 mm NaCl for 12 h and then transferred onto medium containing 125 mm NaCl with 0 or 10 μm ACC for a further 12 h growth. Scale bar = 20 μm. B, Density of cortical microtubules quantified using ImageJ software. Data represent mean ± sd of 3 independent experiments with a minimum of 10 cells from 3 seedlings assessed in each experiment. C, Wild-type (Col-0 ecotype), ACC-pretreated wild-type, wdl5-1, and ACC-pretreated wdl5-1 seedlings were grown for 5 d and then transferred to plates containing 200 mm NaCl and grown for a further 3 d. D, The graph shows the survival rate of seedlings. Data shown are the mean ± sd of 3 independent experiments with a minimum of 80 seedlings assessed in each experiment. E, RT-qPCR analysis of WDL5 transcripts in wild-type, ein3eil1, and 2 ein3eil1 lines expressing pSuper:WDL5-Myc (OE#1/ein3eil1 and OE#2/ein3eil1). F, Wild-type, ein3eil1, OE#1/ein3eil1, and OE#2/ein3eil1 seedlings were grown for 5 d and then transferred to plates containing 200 mm NaCl and grown for a further 3 d. G, The graph shows the survival rate of seedlings. Data represent the mean ± sd of 3 independent experiments with a minimum of 80 seedlings assessed in each experiment. Student’s t test, **P < 0.01.