Interaction of KIN10/11 with STKR1 and subcellular localization of STKR1 in planta. A, Visualization of protein interactions in planta by the BiFC assay. YFP confocal microscopy images show tobacco leaf epidermal cells transiently expressing constructs encoding the fusion proteins indicated. DIC, Differential interference contrast. Bars = 20 µm. B, Coimmunoprecipitation of GFP-STKR1 with KIN10-HA. Both proteins were transiently coexpressed in leaves of N. benthamiana using A. tumefaciens infiltration. After 48 h, total proteins (Input) were subjected to immunoprecipitation (Eluat) with GFP-trap beads followed by immunoblot analysis using either anti-GFP or anti-HA antibodies. C, Subcellular localization of GFP-STKR1 in N. benthamiana leaves transiently transformed by A. tumefaciens infiltration. Bars = 20 µm.