GmZF351 activates the expression of BCCP2, KASIII, TAG1, and OLEO2 by binding to the promoter regions. A, ChIP-seq genome browser views of GmZF351 occupancy at four selected loci in Col-0 and 35S:GmZF351-FLAG transgenic plants. Orange peaks represent read numbers in transgenic line OE-19, and black peaks represent read numbers in Col-0. Bars indicate 1-kb regions in the genome. B, Positions of the promoter sequences in GmZF351-binding genes identified by ChIP-seq. The black lines and blue rectangles indicate 2-kb promoter regions and CDS regions, respectively. The black rectangles indicate promoter fragments used for ChIP-qPCR analysis. C, ChIP-qPCR analysis of the GmZF351-binding genes. Chromatin immunoprecipitated without antibody was used as a negative control, while isolated chromatin before immunoprecipitation was used as an input control. The ChIP signal (% input) was quantified as a percentage of input DNA by qPCR. Asterisks indicate significant differences of anti-FLAG ChIP signal in GmZF351-OE plants compared with those in Col-0 (*, P < 0.05 and **, P < 0.01). Error bars indicate sd (n = 3). D, Expression of BCCP2, KASIII, TAG1, and OLEO2 in GmZF351 transgenic Arabidopsis plants. RNA samples were prepared from 5-d-old seedlings. The mRNA level (relative to At4g12590) of each gene in Col-0 was set to 1. Error bars indicate sd (n = 3). E, GmZF351 activates the promoter activity of BCCP2, KASIII, TAG1, and OLEO2 genes by transient expression assay in tobacco leaves. The promoters of downstream genes are fused with LUC as reporters. The A. tumefaciens harboring reporter vector was mixed with A. tumefaciens harboring 35S:GmZF351-FLAG and infiltrated into tobacco leaves. A. tumefaciens harboring pGWB412 was used as a negative control. LUC images were taken 2 d after infiltration. F, Quantitative analysis of the luminescence intensity in E. Luminescence intensity is analyzed with IndiGo software. Error bars indicate sd (n = 5).