Correlation analysis of GSNO-mediated changes of H3K9/14ac and gene expression. A, qPCR analysis of 14 genes displaying significant changes of H3K9/14ac after GSNO treatment in the ChIP-seq experiment. RNA was isolated from the same samples that were used for the ChIP-seq experiment (n = 2). mRNA levels are expressed as means ± se. Since only two replicates per data point were used, a significance test was omitted. B and C, Correlation analysis of genes displaying GSNO-mediated H3K9/14ac changes after 3 h (B) or 16 h (C) and GSNO-induced transcriptional changes (after 3 h), based on a recently published RNA-seq data set (Begara-Morales et al., 2014). FC, Fold change.