Enhanced levels of JA and JA-Ile in Pi-deficient tissues. A, B, E, and F, JA (A and E) and JA-Ile (B and F) contents in leaves (A and B) and roots (E and F) from plants grown for 12 d in medium containing 1 mm or 100 µm  Pi. C and D, JA (C) and JA-Ile (D) contents in shoots from plants grown for 12 d in medium containing 1 mm or 100 µm  Pi and 1 h after wounding. G to J, JA (G and I) and JA-Ile (H and J) in shoots of 4-week-old soil-grown Col-0, pho1-7, and pho1-2 plants sampled before wounding (G and H) or 1 h after wounding (I and J). For A to F, data are means ± sd of five samples obtained from independent plates, with each sample consisting of a pool of tissues isolated from 20 plants for leaves and 100 plants for roots. For G to J, data are means ± sd of five samples from plants grown in independent pots, with each sample being a pool of three plants. Asterisks denote statistical significance (*, P < 0.05; **, P < 0.01; and ***, P < 0.001) according to Student’s t test. For G to J, statistical analysis was performed comparing Col-0 with each of the pho1 mutants. Experiments were performed independently one time (I and J), two times (C, D, G, and H), or three times (A, B, E, and F) with similar results. FW, Fresh weight.