The phenotype of sls1 is independent of lks1-2 but dependent on AtKC1^D. A, The genotypes of CIPK23 and AtKC1 in different plant materials were confirmed using CAPS markers. The restriction enzymes AflII and ClaI were used to identify the wild-type and mutant forms of CIPK23 and AtKC1, respectively. B, Phenotype comparison among the wild type (Col), lks1-2, sls1 (lks1-2 AtKC1^D), and AtKC1^D after growth on MS or LK medium for 10 d. C and D, K⁺ content measurements in the various plant materials described in B. The K⁺ content was measured after the plants were transferred to MS or LK medium for 10 d. Data are shown as means ± se (n = 4). Student’s t test (#, control, *, P < 0.05 and **, P < 0.01) was used to analyze statistical significance. #, control. E, Real-time PCR analysis of AtKC1 expression in the atkc1/AtKC1^D complementation line. F, Phenotype comparison among the wild type (Col), atkc1, AtKC1^D, and atkc1/AtKC1^D after growth on MS or LK medium for 10 d. G and H, K⁺ contents in the various plant materials described in F. DW, Dry weight.