H3K36ac distribution across genes. A, Metagene plots of H3K36ac enrichment around the TSS (left panel) and TTS (right panel). The highest enrichment was detected within the first 500 bp distal to the TSS. B, H3K36ac enrichment is independent of gene length. Metagene plots are shown for the first (black), second (red), third (green), and forth (blue) length quartile where the black line represents the 25% shortest genes. C, Distribution of gene-specific H3K36ac scores defined as the mean H3K36ac signal within a window of 35 to 516 bp distal to the TSS. Note the two separate populations of genes with scores of less or more than 27, respectively. D, Relation between H3K36ac score and transcriptional state. H3K36ac scores are shown for the 10% genes with lowest (red) and highest (green) mRNA levels, respectively. E, H3K36ac metagene plot around the TSS for the 10% genes with lowest (red) and highest (green) mRNA levels, respectively. Only active genes have the characteristic H3K36ac peak within the first 500 bp. Expression data are from Shu et al. (2012).