Exogenous GA accumulation in the mesophyll is regulated by TEMPRANILLO. A, B, C, D, E, F, G, H, and I, Arabidopsis rosette leaf confocal sections of the first two rosette leaves of 9 DAG plants. A, B, C, GA 3-Fl accumulates exclusively in the mesophyll (A, B) while not in the epidermis (C) of 9 DAG plants. B, C, Images taken in bright field. D, E, GA 3-Fl accumulates with a higher intensity compared with wild type (G) and it is distributed in a much bigger area in the mesophyll of tem1-1 tem2-2 (D), and PCAB3 :amiR TEM (E) rosette leaf of 9 DAG plants. (F) Exogenous Fl used as a negative control only accumulates in the epidermal stomas but not in the mesophyll. G, H, I, GA 3-Fl accumulation in wild type (G), PCAB3 : TEM 2 tem1-1 tem2-2 (H), and PCAB3 : TEM 2 (I) mesophyll of 9 DAG rosette leaves. Scale bars from (A to C) represent 50 μm and from (D to I) represent 100 μm.
