TEMPRANILLO affects trichome number. A, B, and C, SEM of tem1-1 tem2-2 (A), wild-type (B), and P35S:  TEM  2 rosette leaves (C). Scale bars represent 500 μm (A–C). D, E, and F, Inflorescences of the same genotypes showing sepal trichomes. tem1-1 tem2-2 shows more trichomes (A and D) than the wild-type (B and E), whereas P35S:  TEM  2 (C and F) are almost glabrous. G, Trichome number in the 5th-6th rosette leaves of 21 DAG plants of different genetic backgrounds, as indicated. Error bars indicate sd of the mean number of trichomes. Asterisks indicate statistically significant differences (* P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001) obtained using Tukey’s range test. H and I, GUS staining of P  TEM  1:GUS (H) and P  TEM  2:GUS (I) reveals a strong expression of both TEM genes in trichomes. Pictures were taken at same magnification (D–F, H, and I).