OsOTS-RNAi roots are highly sensitive to salt. Root images of vector-only control, OsOTS1-OX, and OsOTS-RNAi seedlings grown in liquid MS medium (in absence of NaCl; A–C) or in presence of 150 mm NaCl (D–F). OsOTS-RNAi showed dramatically reduced root growth. G, Quantification of root length and root fresh weight of 10-d-old plants in MS and MS with 150 mm NaCl. Each column represents an average of five independent biological replicates with 20 seeds in each experiment. Error bars indicate sd. P values for differences between empty vector only and the transgenic lines: *P < 0.05 and **P < 0.001 (two-way ANOVA test).