Phylogenetic analysis of streptophyte and representative chlorophyte CysPc sequences. The CysPc sequences were aligned with MAFFT (version 7.058b). The tree was constructed using RAxML with 1,000 bootstrap replicates using the WAG model with γ-distributed rate heterogeneity. TML calpains cluster in a separate group from the cytosolic calpains. Land plant sequences are highlighted in green, and charophyte and chlorophyte algae sequences are in red and blue, respectively. Cytosolic rat calpains are indicated by capn1 to capn3.