LDH is required in enterocytes for the non-autonomous effect of MPC loss on stem cell proliferation. (A-F) The myo1A-GAL4 driver was used to target RNAi for mCherry (A), dMPC1 (B,E), PDH-E1 (C,F), and LDH (D-F) in the presence of Tub-GAL80^ts. The upd3.1-lacZ reporter (gray) was used to detect Upd3 expression, proliferating ISCs are marked by staining for pHH3+ cells (red), and nuclei are stained with DAPI (blue). Images depict the R4 region of the intestine. Scale bar represents 50 µm. (G) The myo1A-GAL4 driver was used to target RNAi for mCherry (control), LDH, dMPC1, or PDH-E1 in the presence of Tub-GAL80^ts. The number of cells staining positive for phosphorylated histone H3 (pHH3+ cells) was quantified per intestine after shifting 4-5 day old animals to 29°C for seven days. Data are plotted as the mean ± SEM, n ≥ 20 animals for each condition. ****P ≤ 0.0001.