Pectinase unmasking treatments and to examine RGI distribution and evaluate HG-mediated adhesion. A, Immunolabeling of RGI with INRA-RU2. The arrows indicate more intense labeling at the isthmus and lateral bands. B, INRA-RU2 labeling after treatment with PL for 2 h, highlighting nonlabeled gaps (arrows). C, Labeling of arabinan with LM6. D, Labeling with LM6 after PL treatment, highlighting the nonlabeled gaps (arrows). E, LM6 labeling after 2 h of arabinofuranosidase treatment. F to I, Adhesion assay, showing cells adhering to the surface of a plastic petri dish (F) and after pretreatment with PME (G) or PL (H) and after a PL treatment followed by a recovery period in normal growth medium (Rec; I). Bars = 12 µm (A), 8 µm (B), 7 µm (C), 9.5 µm (D and E), and 5 mm (F–I).