The strong phenotype of tpTOC75-DAGK-producing lines is not due to reduction of TOC75 expression. A, RT-PCR results showing levels of TOC75 (At3g46740) or ACT2 (At3g18780) mRNA in five independent tpTOC75-DAGK lines (1–5) or wild-type (WT) plants. The control (C) has no template DNA added. B, Four-week-old seedlings of the wild type or tpTOC75-DAGK grown on 1% Suc-supplemented Murashige and Skoog medium. C, Full rosette leaves of the 6-week-old wild type or tpTOC75-DAGK grown on soil.