Figure 5.
Interrelationship of SnRK1 marker genes and T6P in response to Suc and Glc feeding with full nutrition at 22°C and after transfer to 10°C or zero nitrogen compared with treatment with no supplementary sugar. Marker genes normally up-regulated by SnRK1, ASN1 (A; At3g47340; R  2 = 0.36, SEE = 0.54), βGAL (B; At5g56870; R  2 = 0.50, SEE = 0.41), AKINβ (C; At5g21170; R  2 = 0.60, SEE = 0.44), TPS8 (D; At1g70290; R  2 = 0.59, SEE = 0.45), and TPS10 (E; At1g60140; R  2 = 0.72, SEE = 0.32), and marker genes normally down-regulated by SnRK1, UDPGDH (F; At3g29360; R  2 = 0.64, SEE = 0.18), MDH (G; At3g15020; R  2 = 0.70, SEE = 0.23), TPS5 (H; At4g17770; R  2 = 0.68, SEE = 0.25), and bZIP11 (I; At4g34590; R  2 = 0.62, SEE = 0.28). The inset graphs show the curves for values of T6P between 0 and 0.5 nmol g−1 FW. Horizontal error bars are the sd of three independent T6P samples, and the vertical error bars are the sd of three independent qRT-PCR samples. The fitted lines are significant for each data group (P < 0.001).

Interrelationship of SnRK1 marker genes and T6P in response to Suc and Glc feeding with full nutrition at 22°C and after transfer to 10°C or zero nitrogen compared with treatment with no supplementary sugar. Marker genes normally up-regulated by SnRK1, ASN1 (A; At3g47340; R  2 = 0.36, SEE = 0.54), βGAL (B; At5g56870; R  2 = 0.50, SEE = 0.41), AKINβ (C; At5g21170; R  2 = 0.60, SEE = 0.44), TPS8 (D; At1g70290; R  2 = 0.59, SEE = 0.45), and TPS10 (E; At1g60140; R  2 = 0.72, SEE = 0.32), and marker genes normally down-regulated by SnRK1, UDPGDH (F; At3g29360; R  2 = 0.64, SEE = 0.18), MDH (G; At3g15020; R  2 = 0.70, SEE = 0.23), TPS5 (H; At4g17770; R  2 = 0.68, SEE = 0.25), and bZIP11 (I; At4g34590; R  2 = 0.62, SEE = 0.28). The inset graphs show the curves for values of T6P between 0 and 0.5 nmol g−1 FW. Horizontal error bars are the sd of three independent T6P samples, and the vertical error bars are the sd of three independent qRT-PCR samples. The fitted lines are significant for each data group (P < 0.001).

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