Separation of LOX-dependent branches of the oxylipin pathway based on their ANOVA directions in the time dimension. A, Schematic representation of the three main LOX-based branches of the oxylipin pathway. Free linolenic acid released by the induced activity of a glycerolipase (NaGLA1) is oxygenated by NaLOX3 enzymes (LOX) and converted by a multienzymatic cascade into JA. JA-Ile formed after enzyme-dependent conjugation of JA to Ile and is the bioactive jasmonate interacting with the F-box receptor protein NaCOI1 and NaJAZ transcriptional repressor proteins. C12 and C6 aliphatic chains are produced by the cleavage of NaLOX2-dependent hydroperoxides. Divinyl ether (DVE) oxylipins are produced by enzymatic rearrangement of NaLOX1-dependent hydroperoxides. B, HCA of ANOVA directions for interactive effects for genes involved in the two different branches of the LOX pathway. Elements of the NaLOX3 branch (red) exhibit differential regulation in leaf tissues 1 h after OS elicitation. NaLOX2 and NaLOX1 branches (green) exhibit high interactive effects 5 h after elicitation. C, Temporal profiles for genes from the NaLOX3 pathway showing a high degree of coordination at 1 h and from the NaLOX2 and NaLOX1 pathways showing a high degree of coordination at 5 h.