Time series-dependent multifactorial classification of transcripts and encoded molecular processes. A, We employed a bootstrap-based nonparametric ANOVA model to classify transcript levels based on combined significant responses in different tissues and for different treatments across the time series. Transcripts with no significant changes in expression across different conditions were excluded from further analysis. B and C, Functional categorization of transcripts. GO classification was performed for processes with a bin-specific enrichment statistic of F < 0.05. TCA, Tricarboxylic acid. D, Box plots of ANOVA directions, described as a time-response metric representing the significant interactive effect along the time series while comparing systemic tissues (unelicited leaves and roots) with the treated leaves. E, Temporal profiles of two representative genes (NaLOX3 and NaPMT) obtained from clusters exhibiting interactive effects between treated and systemic tissues using ANOVA model analysis on transcript time-response behaviors.