RT-qPCR analysis of transcript accumulation. Levels of SULTR2, SLT1, SLT2, SULTR1, SLT3, SULTR3, ARS2, PHOX, and NIT1 transcripts were measured in cells deprived of S, N, or P (samples were collected 12 h after transferring cells from nutrient-replete medium to medium devoid of S, N, or P). Levels of individual transcripts are represented as relative abundance with respect to the housekeeping control gene (CBLP).