Expression of plant-type LCY-Bs. Soluble lysate from cells harboring the control vector and lines expressing wild or mutated plant reLCY-B or reCCS was used for affinity purification as described in “Materials and Methods.” Affinity-purified wild and mutant reLCY-B or reCCS were probed with anti-LCY-B or anti-CCS. The arrowheads indicate the signal at approximately 66 kD.