Functional characterization of Ospho1;1 mutants. A, Positions of Tos17 and T-DNA insertions in the OsPHO1;1 gene are indicated by arrowheads. Black boxes represent exons and black lines represent introns. Small arrows represent the gene-specific primers (FL1 and RL1) used for RT-PCR. B, RT-PCR using RNA of roots from mutants and wild-type (WT) plants. C, Morphological appearance of Ospho1;1 null mutants plants compared to the wild type. Plants were pregerminated in water for 7 d and then grown hydroponically for 6 weeks in presence of full Hoagland medium (1 mm Pi), before being harvested. Black bar represents 10 cm. D and E, Fresh weight (FW; D) and Pi content (E) were assessed for shoots and roots of Ospho1;1 mutants and the wild type after 6 weeks of growth in medium with 1 mm Pi. All data are means of six replicates with error bars indicating sd. [See online article for color version of this figure.]