Subcellular localization of plant Inh3. A, Gene constructs encoding sGFP:AtINH3 and CRY2-ΔN:DsRed were cotransformed into Vicia guard cells by particle bombardment, and fluorescent images were obtained by confocal laser-scanning microscopy. The individual fluorescent image, merged image, and corresponding differential interference contrast (DIC) image are presented. Bar = 10 μm. B and C, sGFP:AtINH3 (B) or sGFP:AtINH3-W43A (C) were cotransformed with the mCherry fusion Arabidopsis PP1c (mCherry:TOPP) in Vicia guard cells.