Sequence alignment of plant Inh3. A, Alignment of the deduced amino acid sequences of plant Inh3 from V. faba (VfInh3), Arabidopsis (AtInh3), M. truncatula (MtInh3), and O. sativa (OsInh3a and OsInh3b). Asterisks show identical amino acids. Dashes indicate gaps to allow for optimal alignment of sequences. The sequences were aligned using the ClustalW program (Thompson et al., 1994). Regions corresponding to the RVxF motif are highlighted with a gray background. B, Phylogenetic analysis of Inh3 proteins in plants (V. faba, Arabidopsis, M. truncatula, and O. sativa), protozoa (Plasmodium falciparum), fungi (Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Neurospora crassa), insects (Drosophila melanogaster), nematode (Caenorhabditis elegans), vertebrates (Danio rerio and Xenopus laevis), and mammals (Mus musculus and H. sapiens). The names and accession numbers for individual proteins are shown. Multiple alignments and bootstrap values with 1,000 replicates for this tree are shown in Supplemental Figures S1 and S2, respectively. The bar represents substitutions per site.