Expression of JERF3 in tobacco interacts with the promoters of NtSOD and NtCA in transient GUS assay. A and B, The top section displays a schematic diagram of the reporter constructs used in Agrobacterium-mediated transient expression assay. The promoter of NtSOD-f, NtSOD-p1, NtSOD-p2, NtSOD-mini, or removal of GCC box (GCC-del) is fused upstream of GUS gene in pCAMBIA1303. The promoter of NtCA-p1, NtCA-p2, NtCA-p3, NtCA-p4, NtCA-p5, NtCA-p6, NtCA-p7, NtCA-mini, or removal of 39-bp fragment (NtCA-39 del) is fused upstream of GUS gene in pCAMBIA1303. The chart shows relative GUS activity in leaves of wild-type and OE seedlings 48 h after Agrobacterium infiltration. The data for GUS activity in OE seedling leaves are relative to that in wild type (standardized to 100).