Expression of JERF3 in tobacco enhances the activity of SOD and decreases the accumulation of ROS. A, Expression of JERF3 in tobacco enhances the activities of SOD. The SOD activities were measured based on the ability of SOD to inhibit the reduction of NBT by superoxide. And the data are shown compared to the wild type of control (taken as 100) after calculation of the SOD activities each sample. Results are the average of three replicates. B, Expression of JERF3 in tobacco decreases the accumulation of ROS. The contents of O2 − in tobacco leaves were stained using NBT as substrate. Approximately 15 seedlings in triplicates were used for each line in this assay. Control indicates seedlings raised under normal conditions of growth, Drought indicates seedlings kept on tissue paper for 1 h, Cold represents seedlings exposed to 4°C for 2 d, and ABA and Salt indicate seedlings sprayed with ABA or NaCl, respectively.
