Elemental analysis of eca3 mutant Mn, Ca, and Zn concentration in root and shoot tissue of wild type (black bars), eca3-1 (gray bars), and eca3-2 mutants (white bars) grown on plates on basal (0.5× Murashige and Skoog*) or 0 Mn (0.5× Murashige and Skoog* with Mn omitted) for 17 d. Results are means ± se from four independent pools of plants from different experiments and each pool is from at least five replicate agarose plates. *, Significant difference between mutant and wild-type plant (P < 0.05); Student's t test (paired).