Correlation between amphotericin B (AMB) accumulation and miltefosine exposure in patients receiving combination therapy. AMB accumulation is expressed in terms of D11/D1 AMB AUC_0–24h ratio, while miltefosine exposure is expressed in cumulative area under the contration–time curve until the end of the first treatment cycle (miltefosine AUC_0–D28). The black line indicates the linear regression line (P = 0.0313, R²=0.26), and the grey shaded area the 95% CI. Individual observations are indicated in solid triangles for patients who were parasite negative and open squares for patients still parasitologically positive after one treatment cycle (Day 29). The horizontal dashed line indicates the median D11/D1 AMB AUC_0–24h ratio of 2.4, while the vertical dashed line depicts the median miltefosine exposure AUC_0–D28 at 314 μg·day/mL. Two patients had aberrant sampling schedules and were excluded.