MHZ11 Modulates Lipid Metabolism in Rice Roots.

(A) and (B) MHZ11-related lipids in roots of the wild type (WT), mhz11 mutant, and MHZ11OE-4 transgenic line. Roots of 3-d–old etiolated seedlings treated with or without 10 µL L^-1 of ethylene for 6 h were collected and weighed precisely. Polar lipids were analyzed using a model no. 1260 HPLC system (Agilent Technologies) coupled with a triple quadrupole/ion trap mass spectrometer (QTRAP 5500). Data are means ± sd from four biological replicates (10 seedlings per replicate). Student’s t test indicates a significant difference compared with the corresponding WT control (^*P < 0.05).

(C) Heatmaps of MHZ11-related lipids in the wild-type (WT), mhz11 mutant, and MHZ11 transgenic overexpression line OE-4 according to the lipid profile analysis. Bars indicate the Z-score. ET, ethylene; PI, phosphatidylinositols; LPS, lyso-PS; LPC, lyso-PC; LPE, lyso-PE. Student’s t test indicates a significant difference compared with the corresponding WT control (^*P < 0.05).