Sequencing Analysis of miRNAs and Target RNAs Recovered from Catalytically Active or Inactive AGO1 Complexes.

(A) Flowchart of analytical steps to test immunoprecipitation of AGO1 forms with miRNAs and target transcripts.

(B) Venn diagram showing the association of miRNAs with distinct AGO1 forms. The number of miRNAs present in each category is shown.

(C) Scatterplot of the miRNA/miRNA* ratio from AGO1-DDH and AGO1-DAH for all known miRNAs in the immunoprecipitated fractions. The Pearson correlation coefficient (r) is shown.

(D) Venn diagram showing the association of known AGO1 target sites with distinct AGO1 forms. The number of miRNA target sites present in each category is shown.

(E) Distribution of transcript reads relative to miRNA target sites. Reads from AGO1-DDH and AGO1-DAH input or immunoprecipitated fractions from miRNA target sites (top) or from randomly selected sites (bottom) are plotted by the scrolling window method (20-nucleotide windows, 5-nucleotide scroll). Plots are centered on miRNA target sites (top) or randomly selected sites (bottom) and include 200 nucleotides upstream and downstream.