Effects of Wild-Type and Modified AGO2 Forms on TAS1c-A388T Transcript Targeting and tasiRNA Biogenesis.

Accumulation of TAS1c-A388T transcript and tasiR255 in N. benthamiana leaves from assays testing AGO2 forms. Constructs were coexpressed as indicated above the blot panels. Top, mean (n = 3) relative TAS1c-A388T transcript (light blue) and tasiR255 (dark blue) levels + sd (lane 2 and lane 3 = 1.0 for TAS1c-A388T transcript and tasiRNA255, respectively). amiR173/amiR173-5′A and HA-AGO2 blots are shown as controls. Only one blot from three biological replicates is shown. U6 and l-Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) blots, as well as ethidium bromide–stained rRNA gels, are shown as loading controls. nt, nucleotides.