Cytokine production and macrophage polarisation are normalised by tofacitinib citrate treatment in Ptpn2-LysMCre mice. A] mRNA expression of Il6, Il10, and Il22 normalised to Gapdh in whole proximal colons of mice treated with vehicle or tofacitinib citrate twice daily for 7 days. B] Protein expression of IL-6, IL-10, and IL-22 as determined by ELISA. C] Proportions of total macrophages [CD11b+, Ly6G-, F4/80+, CD64 + cells], M1 [CD86 high, CD206 low], and M2 [CD86 low, CD206 high] macrophages in whole proximal colons of mice as determined by flow cytometry [all cells were pre-gated on live, single, CD45 + cells]; *p ≤0.05, **p ≤0.01, ***p ≤0.001, **** p ≤0.0001; n = 6 per group, representative results of two independent experiments.