(a) Strains used in this study and their antibiotic susceptibilities and genetic profiles. (b) Susceptibility of JE2-derived strains to antibiotics targeting different PBPs and peptide-based antibiotics. Log₂ fold change calculated relative to the MIC for the parent JE2 strain. VAN, vancomycin; DAP, daptomycin; DAL, dalbavancin; NAF, nafcillin; LEX, cefalexin; PBPns, non-selective PBP inhibitor.