Interaction between Rice Esc and E(z) Homologs and Increased H3K27me3 at Target Gene.

(A) Examination of the interaction between FIE1 and rice E(z)-like proteins (left), and FIE2 and rice E(z)-like proteins (right). The interactions were verified by growing the yeast on selective medium [SD/-Leu-Trp-His with 3-amino-1, 2, 4-triazole (3-AT)].

(B) Expression analysis and H3K27me3 status of Os11g31770 gene. The expression of Os11g31770 gene was validated by RT-qPCR (left). H3K27me3 ChIP-seq data for Os11g31770 gene are shown (right), and three subregions (1, 2, and 3) were validated by ChIP-qPCR analysis (middle). The wild type (WT) is shown in red in the top part, and Epi-df is shown in cyan in the middle part. Gene models are shown at the bottom. The two DNA stands are indicated by “+” and “−.” Values are means ± sd of three biological replicates for transcription analysis and two biological replicates for ChIP analysis.