COUP-TFII promotes proliferation, migration, and invasion of ICC cells in vitro    (A) The mRNA expression and protein expression of COUP-TFII in ICC cell lines after COUP-TFII knockdown or overexpression. (B) Cell proliferation was detected by growth curve assay in the indicated cell lines. (C, D) The motility and invasion abilities of ICC cells were determined by transwell assays, and statistics are shown with a bar graph. Scale bar: 100 μm. Data are expressed as the mean ± SD. The results are representative of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.