DNMT3A repressed the transcription of E2F1 target genes through affecting the binding of E2F1 to its recognition sequence within gene promoters (A) Schemes of EphrinB2 and HEY2 promoter constructs with relative locations of predicted E2F1 binding sites. (B) Cos-7 cells were cotransfected with EphrinB2 or HEY2 promoter vector and DNMT3A and/or E2F1 expression plasmids for 24 h, and then luciferase activity was measured. (C) A schematic diagram showing the relative positions of the sites in EphrinB2 or HEY2 promoter. (D) HUAECs were cotransfected with vector, E2F1, DNMT3A, and E2F1/DNMT3A, and ChIP assays were performed using a rabbit IgG as a control and with anti-E2F1 antibody as indicated. Data are presented as the mean ± SD relative to the total DNA input signal, n = 3. *P < 0.05, **P < 0.01.