Knockdown of DNMT3A enhanced the expression of EphrinB2 and HEY2 and stimulated angiogenesis in E2F1-expressing HUAECs    (A) RT-PCR analysis of EphrinB2 and HEY2 in E2F1-expressing HUAECs co-transfected with DNMT3A siRNA or control siRNA. Western blots (B) and quantification (C) of EphrinB2 and HEY2 in E2F1-expressing HUAECs co-transfected with non-targeting shRNA vector or DNMT3A targeting shRNA. Matrigel assay (D) and quantification (E) of E2F1-expressing HUAECs co-transfected with non-targeting shRNA vector or DNMT3A targeting shRNA. (F) Migration of E2F1-expressing HUAECs co-transfected with non-targeting shRNA vector or DNMT3A targeting shRNA detected by wound healing assay. (G) Analysis of wound closure percentage. (H) Proliferation of E2F1-expressing HUAECs co-transfected with non-targeting shRNA vector or DNMT3A targeting shRNA detected by MTT assay. Data were presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01.