Overexpression of DNMT3A suppressed E2F1-induced expression of EphrinB2 and HEY2 and inhibited angiogenesis in HUAECs    (A) RT-PCR analysis of EphrinB2 and HEY2 in HUAECs co-transfected with vector, E2F1 and/or DNMT3A. Western blots (B) and quantification (C) of EphrinB2 and HEY2 in HUAECs co-transfected with vector, E2F1 and/or DNMT3A. Matrigel assay (D) and quantification (E) of HUAECs co-transfected with vector, E2F1 and/or DNMT3A. (F) Migration of HUAECs co-transfected with vector, E2F1 and/or DNMT3A detected by wound healing assay. (G) Analysis of wound closure percentage. (H) Proliferation of HUAECs co-transfected with vector, E2F1 and/or DNMT3A detected by MTT assay. Data were presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01.