Overexpression of E2F1 upregulated the expression of arterial marker genes and promoted angiogenesis in HUAECs    (A) RT-PCR analysis of EphrinB2 and HEY2 in HUAECs transfected with E2F1 or empty vector. Western blots (B) and quantification (C) of EphrinB2 and HEY2 in HUAECs transfected with E2F1 or empty vector. Matrigel assay (D) and quantification (E) of HUAECs transfected with E2F1 or empty vector. (F) Migration of HUAECs transfected with E2F1 or empty vector detected by wound healing assay. (G) Analysis of wound closure percentage. (H) Proliferation of HUAECs transfected with E2F1 or empty vector detected by MTT assay. Data were presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01.