Organization of the artificial gene clusters used for production of plant-specific compounds in E. coli. All three genes contained their own T7 promoter and RBS. Schematic representation of the strategies used for construction of pET-T5 M (a), pET-TLC, and pET-TLkS (b). All constructs contained the T7 promoter and the RBS in front of each gene, and the T7 terminator located in the rear of each gene. The following abbreviations are used: B, BamHI; Bg, BglII; H, HindIII; N, NdeI; X, XhoI; P, PacI; S, SpeI; Tp, T7 promoter; T_T, T7 terminator; RBS, ribosomal binding site