Fig. 8
Effects of RCS scavengers on MeJA-induced [Ca2+]cyt elevations in Arabidopsis guard cells. Representative yellow cameleon 3.6 fluorescence emission ratios (535 nm/480 nm) showing [Ca2+]cyt elevations in Arabidopsis guard cells in response to the indicated treatments: (A) 0.1% ethanol (n = 22), (B) 10 µM MeJA (n = 22), (C) 10 µM MeJA + 1 mM carnosine (n = 22), (D) 10 µM MeJA + 0.5 mM pyridoxamine (n = 20), (E) 1 mM carnosine (n = 20) and (F) 0.5 mM pyridoxamine (n = 22). (G) Percentage bar chart showing the number of control (0.1% Ethanol)-, MeJA-, MeJA + carnosine-, MeJA + pyridoxamine-, carnosine- and pyridoxamine-induced [Ca2+]cyt elevations in the Arabidopsis guard cells. The [Ca2+]cyt transient elevations were counted when changes in [Ca2+]cyt ratios were ≥0.1 units from the baseline.