Concentration of Pneumocystis jirovecii in the extracted DNA from oropharyngeal washes (OPWs) of colonized (polymerase chain reaction [PCR] positive/bronchoalveolar lavage [BAL] or induced sputum [IS] negative) versus infected (PCR positive/BAL or IS positive) samples. The figure shows a box and whiskers plot of the concentration of P jirovecii in the extracted DNA from colonized and infected individuals as determined by the dhps real-time PCR. The box represents the 25th to 75th percentile, and the whiskers represent the minimum and maximum values (t test; P > .05). The major surface glycoprotein PCR data were not analyzed in a similar fashion because there were insufficient numbers of PCR-positive samples in colonized patients, and no standard curve was included in the PCR to allow for accurate qua.jpegication.