Hepatitis C virus (HCV)-specific CD8⁺ T cells exhibit a partial reduced exhaustion phenotype following treatment in comparison to baseline values. (A) Flow cytometry gating strategy was used to identify HCV-specific CD8⁺ T cells. Of the 40 participants in our cohort, 17 individuals with HLA-A24:02-1031 (AYSQQTRGL; HCV NS3) were utilized for the analysis. (B) For PD-1, the line graph of marker percentages compares individual differences between pretreatment (PreT) and 12-weeks following sustained virologic response (SVR12). (C) For TIM-3, a mouse Ig1 isotype control was used to determine background. The line graph of marker percentages compares individual differences between PreT and SVR12 values. (D) For TIGIT, the line graph of marker percentages compares individual differences between PreT and SVR12 values. For B–D, green lines indicate negative differences (positive immune outcome) from PreT–SVR12, red lines indicate positive differences (negative immune outcome), and grey lines indicate unchanged differences (less than ± 1 standard deviation change). The black diamonds at both PreT and SVR12 represent the mean value among all participants. Comparisons made between PreT and SVR were assessed via paired t tests. Samples were determined to be statistically different if P < .05.