Figure 4.
![PCR analysis reveals a significantly suppressed FOXP3 expression in the colon and spleen [but not mesenteric lymph nodes or thymus] of IL-6−/−/10−/− mice compared to that in IL-10−/− mice. [A] PCR products. [B] Qualitative analyses of selected genes (only genes whose expression was significantly altered [marked by arrows] are shown). Double arrows indicate the size-shifts [two different sizes] of the PCR products of RORγt in IL-6−/−/10−/− mice, compared to the single-sized band in IL-10−/− mice. [C] Expression of several major cytokines. Representative data from at least 4–6 mice per group [3 months old] are shown. *p < 10–4.](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ecco-jcc/14/6/10.1093_ecco-jcc_jjz176/1/jjz176f0004.jpeg?Expires=1749682269&Signature=20iKQacgN50MIaH1pruxwV1GskKGeI-kbklcJmJtqohAaxdoHbn2GlN-fuN4GWbxDjPsTntPqSK9lhVoICOOLamQ1RIGbbVp7PuNkR7duaPpSdANakeuALCiJUc8kW~Avf~BSi3Zv8lJTylL941uPEavW7j9l--qn3wcKvySUbKy-hgaKgfxkiX1N5Y0KIl~fgcflrITFHqB0c6XcYtPvporcArnUeLEGkfetL~wFYaLtCCrxeq~i5zKp84wht5SyhfrDbFQ-6KCscq9sa7eT63nAKTLMaUQgQnqJd8QWRmT6-qXPUbPOC562yclYPCz1bxq5WA2UlETbdF~pMh7qA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PCR analysis reveals a significantly suppressed FOXP3 expression in the colon and spleen [but not mesenteric lymph nodes or thymus] of IL-6−/−/10−/− mice compared to that in IL-10−/− mice. [A] PCR products. [B] Qualitative analyses of selected genes (only genes whose expression was significantly altered [marked by arrows] are shown). Double arrows indicate the size-shifts [two different sizes] of the PCR products of RORγt in IL-6−/−/10−/− mice, compared to the single-sized band in IL-10−/− mice. [C] Expression of several major cytokines. Representative data from at least 4–6 mice per group [3 months old] are shown. *p < 10–4.
