(a) Full-length alignment of four F1:A2:B20 plasmids harboured by CTX-M-27-producing E. coli strains of ST131 and clade C1-M27, identified in Brazil (pMO, GenBank accession number: MG886288), Sweden (p4_4.1, 125.877, GenBank accession number: CP023827), the United Arab Emirates (pEC-81009, GenBank accession number: CP021180) and Germany (pH105, 134.899, GenBank accession number: CP021871). Arrows indicate the direction of transcription, whereas the grey bands denote regions of identity. Overall, the identity was more than 95%. (b) Schematic representation of the genetic environment of bla_CTX-M-27 genes carried by the pMO and p6M plasmids identified in CTX-M-27-producing E. coli strains, isolated from areas impacted by intensive maritime traffic and transoceanic shipping activities, in Brazil. Arrows indicate the direction of transcription of bla_CTX-M-27 genes (black arrow), genes related to mobile elements (white arrows) and tonB (related to energy transduction functions) and ORF genes (grey arrows).