AGO5–miR156 interaction controls flowering time. Phenotypes of Col-0, ago5 T-DNA insertion mutants (ago5-1 and ago5-5), 35S::miR156 and 35S::miR156/ago5-5 plants. (A) Whole plant phenotypes. Photos were taken at 30 dpg. (B) Number of mature leaves (n = 23 plants). (C) Number of juvenile leaves (n = 23 plants). (D) Days-to-bolting of the aforementioned genotypes was evaluated when bolts were 1 cm tall (n = 23 plants). Experiments were repeated at least three times, and similar results were obtained. Asterisks indicate significant differences from Col-0 in a Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Error bars indicate standard deviation.